Article Dans Une Revue Biochemistry Année : 1996

Topography of the Escherichia coli Initiation Factor 2/fMet-tRNAfMet Complex as Studied by Cross-Linking

Gulnara Yusupova
Joseph Reinbolt
  • Fonction : Auteur
Hiroshi Wakao
  • Fonction : Auteur
Soumaya Laalami
  • Fonction : Auteur
Marianne Grunberg-Manago
  • Fonction : Auteur
Pascale Romby
Bernard Ehresmann
  • Fonction : Auteur
Chantal Ehresmann
  • Fonction : Auteur

Résumé

trans-Diamminedichloroplatinum(II) was used to induce reversible cross-links between Escherichia coli initiation factor 2 (IF-2) and fMet-tRNAfMet. Two distinct cross-links between IF-2 and the initiator tRNA were produced. Analysis of the cross-linking regions on both RNA and protein moieties reveals that the T arm of the tRNA is in the proximity of a region of the C-terminal domain of IF-2 (residues Asn611−Arg645). This cross-link is well-correlated with the fact that the C-domain of IF-2 contains the fMet-tRNA binding site and that the cross-linked RNA fragment precisely maps in a region which is protected by IF-2 from chemical modification and enzymatic digestion. Rather unexpectedly, a second cross-link was characterized which involves the anticodon arm of fMet-tRNAfMet and the N-terminal part of IF-2 (residues Trp215−Arg237).

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hal-05259817 , version 1 (15-09-2025)

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Citer

Gulnara Yusupova, Joseph Reinbolt, Hiroshi Wakao, Soumaya Laalami, Marianne Grunberg-Manago, et al.. Topography of the Escherichia coli Initiation Factor 2/fMet-tRNAfMet Complex as Studied by Cross-Linking. Biochemistry, 1996, 35 (9), pp.2978-2984. ⟨10.1021/bi9519415⟩. ⟨hal-05259817⟩

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